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Construction of a universal recombinant expression vector that regulates the expression of human lysozymein milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract: A key component in the technology is the construction of an efficient milk expression vector.Here, we established a simple method to construct a milk expression vector, by a combination of homologousvector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligationOur results have provided a simple approach to construct a universal milk expression vector, and demonstratedthat the resulting vector regulates the expression of hLZ in milk.

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: One potential method is to produce transgenic livestock that make modified milk containing high concentrationExpression analysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary glandof the transgenic mice and the concentration of rhBCHE in the milk of individual mice ranged from 76

Keywords： butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF BAC)     transgenic mice     milk    

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Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 141-147 doi: 10.1007/s11684-009-0041-6

Abstract: After 72 hours, the expression of GFP could be detected in BV-2 cells.Western blotting revealed that the Rab9 gene expression in BALB/c mice brain was up-regulated significantlyLentivirus encoding Rab9 can increase the expression of Rab9 gene effectively, which might offer a novel

Keywords： Rab9     lentivirus     gene therapy     gene transfer    

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Construction of eukaryotic expression vector of human arresten gene and its secreted expression in HEK

Wei LI PhD , Siming GUAN MM , Zifang SONG PhD , Qichang ZHENG PhD , Jun XIONG PhD , Dan SHANG PhD , Xiaogang SHU PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 297-302 doi: 10.1007/s11684-009-0058-x

Abstract: The eukaryotic expression vector of human arresten gene was constructed and its secretive expressionThe target fragment was inserted into the I and I restriction sites of eukaryotic expression vectorrecombinant plasmid was subsequently transfected into HEK 293 cells with LipofectAMINETM2000 Reagent, and the expression

Keywords： angiogenesis inhibitor     arresten     eukaryotic expression     HEK 293 cells     endothelial cells    

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Construction and expression of hepatitis B virus vector encoding TC-tagged core protein

Yuanyuan LIN MD, Xiaoming CHENG MS, Yuhu SONG MD, Peiyuan LI MD, Ying CHANG MM, Jinjian YAO MD, Jusheng LIN MD, PhD, Li ZHOU PhD, Leiming XU PhD,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 396-402 doi: 10.1007/s11684-009-0056-z

Abstract: In this study, we constructed a recombinant HBV vector encoding TC-tagged core protein for biarsenicaland hepatitis B e antigen (HBeAg) could be expressed in cells transfected with the recombinant HBV vector, which is similar to the cells transfected with wild-type HBV vector.the genetic insertion of TC tag into core protein in some degree, but cells transfected with the HBV vectorTaken together, the recombinant HBV vector can serve as a useful tool to produce HBV virions incorporated

Keywords： hepatitis B virus     vector     tetracysteine tag     core protein    

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Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 10, doi: 10.1007/s11783-022-1541-8

Abstract: ListItem>

● Perfluorooctanesulfonic acid and perfluorooctanoic acid highest in human milk

Keywords： Human biomonitoring     Human breast milk     LC-MS/MS analysis     Lifestyle parameters    

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Global warming potential associated with Irish milk powder production

William Finnegan, Jamie Goggins, Aksana Chyzheuskaya, Xinmin Zhan

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 3, doi: 10.1007/s11783-017-0949-z

Abstract: Therefore, in this paper, a single agri-food product, milk powder, is examined in order to estimate theA cradle-to-processing factory gate analysis, which includes raw milk production, raw milk transportationThe factories surveyed processed approximately 24% of the total raw milk processed in the Republic ofThe average total GWP associated with the manufacture of milk powder is 9.731 kg CO eq·kg milk powder, which has a standard deviation of 2.26 kg CO eq·kg milk powder, for the life cycle stages analyzed

Keywords： Dairy     Global warming potential     Ireland     Life cycle assessment     Milk powder     Milk production    

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Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 287-291 doi: 10.1007/s11684-009-0050-5

Abstract: in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAi) was used to silence the expressionsynthesis and annealing, double strand oligonucleotides were cloned into a linearized pSIH1-H1-copGFP shRNA vectorthat three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vectorLentivector-mediated RNAi could efficiently down-regulate the expression of the murine cdk4 gene , which

Keywords： cyclin-dependent kinase 4     RNA interference     plasmid     lentiviral vector    

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UsingKinect for real-time emotion recognition via facial expressions

Qi-rong MAO,Xin-yu PAN,Yong-zhao ZHAN,Xiang-jun SHEN

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 4,   Pages 272-282 doi: 10.1631/FITEE.1400209

Abstract: In this paper, we propose a real-time emotion recognition approach based on both 2D and 3D facial expressionTo capture the deformation of the 3D mesh during facial expression, we combine the features of animationprofiles (IEPs) and maximum confidence is proposed to recognize emotions with these real-time facial expression

Keywords： Kinect     Emotion recognition     Facial expression     Real-time classification     Fusion algorithm     Support vector    

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CH4 and N2O emissions from double-rice cropping system as affected by Chinese milk

Heshui XU,Dengyun LI,Bo ZHU,Kai ZHANG,Yadong YANG,Chen WANG,Ying JIANG,Zhaohai ZENG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 59-68 doi: 10.15302/J-FASE-2017137

Abstract: Chinese milk vetch (CMV) and rice straw (RS) were incorporated into soil to substitute for synthetic

Keywords： Chinese milk vetch     CH4     double-rice cropping system     grain yield     N2O     rice straw    

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Liquefaction prediction using support vector machine model based on cone penetration data

Pijush SAMUI

Frontiers of Structural and Civil Engineering 2013, Volume 7, Issue 1,   Pages 72-82 doi: 10.1007/s11709-013-0185-y

Abstract: A support vector machine (SVM) model has been developed for the prediction of liquefaction susceptibilityThe effect of capacity factor ( ) on number of support vector and model accuracy has also been investigated

Keywords： earthquake     cone penetration test     liquefaction     support vector machine (SVM)     prediction    

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A review of traditional and novel detection techniques for melamine and its analogues in foods and animal feed

Mengshi LIN,

Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 4,   Pages 427-435 doi: 10.1007/s11705-009-0244-z

Abstract: safety incidents, including 2007 pet food recalls in North America and 2008 melamine contamination in milk, infant formula, and other milk-derived products in China.

Keywords： milk-derived     Melamine     labor-intensive     cyanurate     time-consuming    

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A modified neural learning algorithm for online rotor resistance estimation in vector controlled induction

A. CHITRA,S. HIMAVATHI

Frontiers in Energy 2015, Volume 9, Issue 1,   Pages 22-30 doi: 10.1007/s11708-014-0339-1

Abstract: Online estimation of rotor resistance is essential for high performance vector controlled drives.

Keywords： neural networks     back propagation (BP)     rotor resistance estimators     vector control     induction motor    

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Unconfined compressive strength prediction of soils stabilized using artificial neural networks and support vector

Alireza TABARSA, Nima LATIFI, Abdolreza OSOULI, Younes BAGHERI

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 2,   Pages 520-536 doi: 10.1007/s11709-021-0689-9

Abstract: Two artificial-intelligence-based models including artificial neural networks and support vector machinesThis study demonstrates the better performance of support vector machines in predicting the strengthThe type of kernel function used in support vector machine models contributed positively to the performance

Keywords： unconfined compressive strength     artificial neural network     support vector machine     predictive models     regression    

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Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 166-170 doi: 10.1007/s11684-008-0030-1

Abstract: This study aims to demonstrate that blocking the receptor-interacting protein2 (Rip2) expression canRip2 small interfering RNA (siRNA) plasmids were constructed and transfected into macrophage and Rip2 expressionRip2 siRNA plasmid could block the mRNA and protein expression of Rip2 and promote cell proliferationThe HMGB1 expression in the liver decreased to (40.21 ± 11.03) pg/g, and serum TNF-?The results demonstrate that Rip2 siRNA plasmid can block the expression of Rip2, decrease the production
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Title Author Date Type Operation

Construction of a universal recombinant expression vector that regulates the expression of human lysozymein milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Journal Article

Construction of eukaryotic expression vector of human arresten gene and its secreted expression in HEK

Wei LI PhD , Siming GUAN MM , Zifang SONG PhD , Qichang ZHENG PhD , Jun XIONG PhD , Dan SHANG PhD , Xiaogang SHU PhD ,

Journal Article

Construction and expression of hepatitis B virus vector encoding TC-tagged core protein

Yuanyuan LIN MD, Xiaoming CHENG MS, Yuhu SONG MD, Peiyuan LI MD, Ying CHANG MM, Jinjian YAO MD, Jusheng LIN MD, PhD, Li ZHOU PhD, Leiming XU PhD,

Journal Article

Perfluoroalkane acids in human milk under the global monitoring plan of the Stockholm Convention on Persistent

Journal Article

Global warming potential associated with Irish milk powder production

William Finnegan, Jamie Goggins, Aksana Chyzheuskaya, Xinmin Zhan

Journal Article

Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Journal Article

UsingKinect for real-time emotion recognition via facial expressions

Qi-rong MAO,Xin-yu PAN,Yong-zhao ZHAN,Xiang-jun SHEN

Journal Article

CH4 and N2O emissions from double-rice cropping system as affected by Chinese milk

Heshui XU,Dengyun LI,Bo ZHU,Kai ZHANG,Yadong YANG,Chen WANG,Ying JIANG,Zhaohai ZENG

Journal Article

Liquefaction prediction using support vector machine model based on cone penetration data

Pijush SAMUI

Journal Article

A review of traditional and novel detection techniques for melamine and its analogues in foods and animal feed

Mengshi LIN,

Journal Article

A modified neural learning algorithm for online rotor resistance estimation in vector controlled induction

A. CHITRA,S. HIMAVATHI

Journal Article

Unconfined compressive strength prediction of soils stabilized using artificial neural networks and support vector

Alireza TABARSA, Nima LATIFI, Abdolreza OSOULI, Younes BAGHERI

Journal Article

Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

Journal Article